The Tigers shot a 24-over-par total of 312 on Saturday at Ohio State University's Scarlet Course to finish with a three-day total of 58-over-par 922.
The SEC champs came into the event as the number two seed behind Southern Cal, which won the tournament with a total of 874, which was 25 strokes better than runnerup Vandy.
The top eight teams move on to the NCAA Championships, but Auburn won't be in that group despite coming into the final round in sixth place. On Saturday all five Auburn players struggled as the Tigers dropped to 12th place, nine shots behind Arkansas, the final qualifier.
"It was just not our day," Coach Kim Evans. "The girls really wanted this. We started out alright, but we let the course get to us too much.
"We missed out on a couple shots and then got too aggressive and this is not a course you can get too aggressive on, it will get you," Evans added.
Senior Haley Wilson led the Tigers with a five-over-par 77. The Tigers also counted rounds of 78 from Victoria Trapani and Marta Sanz plus a 79 from Patricia Sanz. An 83 by Carlie Yadloczky did not count in the team total.