The 17th-ranked Cyclones out-rebounded the visitors 47-36 and hit 13 three-pointers to just six for Auburn.
After a solid start the Tigers went cold shooting the basketball while the Cyclones warmed up and took control of the contest midway through the first half.
Iowa State improved to 6-0 with the win while Auburn fell to 4-2, snapping a three-game winning streak for Coach Tony Barbee's Tigers, who were playing on the road for the first time this season.
"We got off to a good start and then we went cold," Barbee said. "It's not what you do on offense, it is what you do on the defensive end of the floor."
The Auburn coach said Iowa State showed much patience offensively and was able to get better shots. The Cyclones made 45.2 percent of their field goals while Auburn hit just 35.5 percent. The Tigers were 6-23 on threes while ISU made 13-35.
Barbee noted that the Cyclones dominating the assist statistic 28-7 was a telling number and added, "The bottom line is they out-toughed us," coach stated.
Senior guard Chris Denson 27 points for the Tigers while junior guard K.T. Harrell added 19 points, but no other Auburn player scored in double figures. Auburn's number three scorer in the contest, junior point guard Malcolm Canada, added seven points and Taj Shamsid-Deen scored six.
Denson hit 8-17 field goals, but was 0-3 on treys. The lefty made 11-13 free throws as the Tigers made 20-24 shots at the foul line.
Dustin Hogue led six Cyclones, who placed six players in double figures in the scoring column. The six-foot-six juco transfer scored a season-high 20 points and pulled down 18 rebounds, which is also a season-best. He made 8-10 field goals and pulled in a dozen defensive rebounds to the delight of a crowd of 13,889 watching the action on Iowa State's campus.
Auburn led most of the first 10 minutes as both teams tried to find the range with their shooting. The Tigers' biggest lead came at 12-5 with 14:55 to go in the half on a three-pointer by Harrell.
Iowa State took the lead for good at the 10:08 mark at 16-15 on a layup by Monte Morris.
Auburn's inability to rebound and stop the Cyclones from three-point range allowed the home team to quickly build the lead to 27-17 at the 8:03 mark. By halftime the Tigers found themselves in a major hole trailing 47-28. Auburn never made a run on the home team in the second half.
Halftime Numbers: The Cyclones out-rebounded the Tigers 30-17 in the period. ISU hit 16-39 field goals, 7-19 threes and 8-11 free throws. Auburn hit 8-30 field goals, 2-11 threes and 10-11 free throws.
Denson led the Tigers in first half scoring with 14 points while Harrell added nine. Hogue had a big first half for the home team with 14 points and 12 rebounds.
Stat of the Game: The Tigers finished the game with 14 turnovers and seven assists while Iowa State had just nine turnovers to go with 28 assists.
Worth Noting: Freshman Matthew Attewe made his Auburn debut after missing the previous games with a leg injury. The 6-9, 250-pounder did not score and he pulled in two rebounds before fouling out after playing for five minutes.
Auburn returns to action on Sunday vs. Illinois in a game played at Philips Arena in Atlanta. Tipoff is set for 2 p.m. CST.