Kukors had the fastest split time with a 1:59.57 on leg two. Ohlgren on leg three swam 200 meters in 2:00.19.
Cesar Cielo, who was named the top swimmer at the 2007 NCAA Men's Swimming and Diving Championships, won a gold medal in the 100-meter freestyle.
The member of AU's national championship team won the 100 meters freestyle while swimming for Brazil and did it in a Pan Am Games record time of 48.79. Former Tiger George Bovell, swimming for Trinidad & Tobago, took seventh.
Also on Wednesday, former Auburn All-American Mark Gangloff took a silver medal in the 100 breaststroke for Team USA in a time of 1:01.24, fourth-tenths off the pace of Canada's Scott Dickens.
Ten current or former Tiger swimmers are competing in the Pan Am Games.