Colorado (16-9, 6-6 Big 12) has won 10 straight at home, tying a school record set in 1979-80, and four of six overall.
Stephane Pelle had 18 points and 18 rebounds, David Harrison added 15 points and 11 rebounds, and Michel Morandais chipped-in 17 points and 10 assists as Colorado placed three players with double-doubles. CU improved its home record to 12-1 overall and 6-0 in the conference.
Rickey Paulding had 22 points and Arthur Anderson 17 to lead Missouri, which lost for the third time in eight games.
Missouri trailed 47-35 early in the second half, but went on a 13-3 run to cut the lead to 50-48, capped by Anderson's jumphook in the lane with 13:34 left.
The Tigers stayed within four midway through, but Colorado went on a 13-4 run and pushed the lead to 71-58 on two free throws by Morandais (six rebounds) with 5:43 left.
Paulding followed with a hanging three-point play, but Wilson answered with a 3-pointer from the wing to give the Buffaloes a lead that would stay in double digits.
Wilson was 6-for-10 from long range and had four steals.
Harrison struggled in the first half but came alive to start the second.
He opened 0-for-5, including a tough shot in traffic to open the second half, but got himself going with a running scoop shot a minute later.
Harrison followed with a three-point play off an offensive rebound, then scored on a tough turnaround in the lane that put the Buffaloes up 56-49 with 13 minutes left. He capped the win with a thunderous two-handed dunk with 41 seconds left that left the rim shaking for nearly a minute.
Harrison also blocked two shots to set a school record with 75 this season.
Missouri's efforts to stop Harrison in the first half worked - he was 0-for-4 with one point - but it left others open.
Wilson took advantage from the outside, hitting 3-of-5 from 3-point range for 15 points in the first half. Pelle worked the opposite of the paint from Harrison for 10 points and 14 rebounds - five offensive.
Neither team led by more than four points until Colorado went on a 10-3 run, capped by Morandais' two free throws with 4:00 left.
Missouri made six of its first nine shots, but had just one field goal - a power move by Johnson - in a span of six minutes as Colorado built a 40-30 halftime lead.
Poncho Hodges held the previous Colorado record for blocked shots in a season with 73 in 1991-92.