The game was dubbed by one fan as "The Singler Mingler." The brothers grew up in Medford, Ore., about 275 miles to the south of where the game was played at the Rose Garden.
The Blue Devils open 6-0 for the fourth straight season, and 18th overall, under Mike Krzyzewski. The win extended Duke's winning streak to 16 games.
E.J. Singler, a sophomore forward, scored 14 points, all in the second half, for Oregon (4-2).
It was the first time the Singlers played against each other. It was the first time they were on the same court in a real game since they led South Medford High School to the state championship in 2007.
The two shared a quick hug and a pat on the back to start the game.
Kyle Singler, the 6-foot-8 big brother, was named the most outstanding player of last year's Final Four, leading the Blue Devils to the national championship.
He opened scoring with a fall-away jumper and Duke never trailed. His 3-pointer midway through the first half put the Blue Devils up 20-7.
Duke went up 31-12 in the first half on Miles Plumlee's alley-oop dunk. The Ducks narrowed it to 33-23 late in the first half, but Duke led 40-25 at halftime.
E.J. Singler had two fouls and no points in the first half. His lone attempt from the field was an airball.
The younger Singler was averaging 11.6 points and 5.2 rebounds going into the game.
E.J. Singler was lured to Oregon by longtime coach Ernie Kent, an alum who was dismissed after the team finished 16-16 last season. The Ducks replaced Kent with Dana Altman, who spent 16 seasons at Creighton.
Kyle Singler hit a 3-pointer to give the Blue Devils a 52-31 lead early in the second half as Duke put it out of reach of the outmatched Ducks. Nolan Smith's fastbreak layup gave Duke a 61-31 lead.
The Rose Garden crowd gave Kyle Singler a standing ovation when he left the game with just over five minutes to play. He raised a hand in acknowledgment and the blue-clad crowd behind Duke's bench chanted his name.
Kyle Singler said Krzyzewski suggested a West Coast game last year and the elder Singler suggested he'd like to play his little brother.
The elder Singler went to Duke the same year that Kevin Love, another Oregon prep star, decided to attend UCLA.
While Love went to the NBA after only a year with the Bruins, Kyle Singler has remained at Duke, where he's averaged 15.8 points and 6.8 rebounds over his career.
This season the elder Singler was averaging 13.4 points and 5.4 rebounds for the Blue Devils, who were coming off an 82-68 victory over No. 4 Kansas State.
The Ducks, coming off a 75-52 victory over Texas Southern on Tuesday, were led by Joevan Catron with 18 points and eight rebounds.
Smith added 18 points and six rebounds for Duke.
It was the first meeting between the schools. The game was well attended by Duke fans, and many of those at the game sported the gear of both teams.