Lee Catchpole set up Martin John five yards in from the net for the game's only goal.
The teams each had six shots and three shots on goal. The difference, Chris Torres stopped two of those attempts.
Duquesne is 4-2-3 while Buffalo raised its overall record to 9-2-1 and its winning streak to six games.
The Dukes will return to Buffalo, N.Y. for their final non-conference matchup against Canisius (2-6-1) on Friday, October 3, 2008, at 3:30 p.m. The Griffs lost to Robert Morris today, 3-0; DU beat the Colonials by a 1-0 score Friday, in overtime.