Senior left fielder Mike Carroll led Duquesne's 15-hit attack with a five RBI, two hit, including a grand slam home run, and two walk outing while scoring in all four chances at the plate.
Senior second baseman Bill Torre, senior center fielder Brian Bernardo and redshirt freshman designated hitter Chris Kimmeth each had two hits. Also homering where Bernardo and sophomore second baseman Andrew Heck, who had four RBI.
The Dukes are now 1-5, 5-22 overall while Charlotte comes in at 1-6, 15-11.
The Dukes opened the scoring with four runs in the bottom of the first on Rick Devereaux' run scoring double, a hit-batsman, a ground-out and Bernardo's RBI single.
Charlotte cut the lead to one in their two-homer, three-run second at bat. Bernardo's lead-off home run in the fourth extended the Dukes' lead to 5-3. Heck's three-run homer in the fifth upped the edge to 8-3.
Duquesne added three more in the bottom of the sixth after Justin Wilson's two-run round tripper in the top of the frame. RBI singles by Carroll. Kimmeth and junior Mark Tracy, a Pepperdine transfer, put the home team in front 11-5.
Charlotte tallied four the rest of the way to match Carroll's bases loaded shot in the seventh for the 15-9 final.
Sophomore Andrew Smith (2-5) picked up the loss.
The teams resumed play in the DH nightcap with senior Gary Pierpont on the mound for the Dukes and junior Kelly McLain in charge for the 49ers.