Florida's final record was 29-28, finishing one-game above the mandated .500 record for a berth.
Kevin O'Sullivan became the first Florida coach to lead the program to six straight NCAA Tournaments.
The Gators will open NCAA Tournament play on Friday at 1 p.m. against Austin Peay. Indiana will face Valparaiso that night. Austin Peay currently leads the nation with a 15-game winning streak.
The winner of the Indiana Regional will go on to meet the winner of the Florida State Regional for Super Regionals the following weekend, with a berth in the College World Series on the line. Alabama, Savannah State and Troy will also participate in the Florida State Regional.