"I knew going in about the chance for the record, but to tell you the truth I completely forgot about it as soon as I stepped on the court," Isner said. "Then when it was 5-2 in the second set, it hit me and I thought to myself that I better go ahead and lock it down now.
"It is a huge honor breaking this record because there have been so many unbelievable players here. For my name even to be mentioned with these guys is an honor. This is very special and still kind of surreal to me."
Isner's teammates Luis Flores and Travis Helgeson also captured victories in the Round of 64. Helgeson won in straights sets over Henrique Concado of Old Dominion, while Flores came back to beat Haythem Abid of UCLA in three sets. Matic Omerzel also competed in the singles draw, but dropped a three-set contest to Matko Maravic of Michigan.
All top eight seeds advanced on the men's side, as did all the Nos. 9-16 except for three. Daniel Vallverdu of Miami upset Baylor's Lars Poerschke and Radford's Martin Sayer knocked off Adam Homstrom in tight straight-sets battles. LSU's Ken Skupski used a dominating performance to upset Todd Paul of Wake Forest, 6-2, 6-3.
In women's action, Georgia senior Natalie Frazier cruised through her first-round opponent in straight sets. She defeated Zuzana Cerna of Baylor, 6-1, 6-2. It was Frazier's first NCAA Singles Tournament victory, as she dropped Round of 64 contests in both 2005 and 2006.
The women's team's other participant almost pulled off a big upset. Monika Dancevic split sets with top-seeded Audra Cohen and was leading 2-0 in the third and on serve. But Cohen stormed back and won six straight games to clinch the match and stay alive in the tournament.
All top eight seeds also advanced in the women's bracket with three upsets of Nos. 9-16 seeds as well.
Singles play resumes Thursday with Round of 32 action at 10 a.m. followed by Round of 32 doubles beginning at 2 p.m.
Two Georgia doubles teams are slated for competition on the men's side. Top-seeded Isner and Flores will face Dan Hanegby and Saurabh Kohli of Brown at 5:30 p.m. and Helgeson and Omerzel will play No. 2-seeded Somdev Devvarman and Treat Huey of Virginia at 6:30 p.m. For the Georgia women, Yvette Hyndman and Darya Hyndman will compete as a No. 5-8 seed against Bianca Dulgheru and Sylvia Kosakowski of Pepperdine at 2:30 p.m.
Isner continues his singles run at noon against Bassam Beidas of Pepperdine. Flores will play Harel Srugo of Old Dominion at 11:30 a.m., and Helgeson will battle California's Pierre Mouillon at 11:30 a.m. Natalie Frazier also continues her quest for a singles title versus Katrina Zheltova of Sacramento State at 1:30 p.m.