The Wildcats dropped to 2-5 against in-state competition on Tuesday after falling 5-4 to Eastern Kentucky at Cliff Hagan Stadium.
Kentucky coughed up a 4-0 lead to the Colonels, who rallied to the lead with three runs in the fifth inning and two in the sixth after being no-hit by Zach Logue for the first four frames.
Mandy Alvarez and Ben Fisher each had two hits to lead the Colonels. Alvarez also drove in the go-ahead run with a sacrifice fly in the sixth inning. Cole Warrenfeltz delivered RBI groundouts in each of the scoring innings for EKU.
Zach Pop (0-2) took the loss for the Cats, surrendering two runs on two hits and a walk in one inning of relief. He also uncorked a pair of wild pitches that helped EKU plate a run in the fifth.
Meanwhile, EKU's one-man bullpen was sensational. Chris Cervantes (1-2) tossed six shutout innings in relief of starter Nick Cumpston, who allowed four runs on five hits and two walks over three innings on the mound.
Junior third baseman JaVon Shelby went 2-for-4 with his 10th home run of the season for UK (27-18) but it wasn't enough to save the Cats from a potentially costly defeat. It marked the 10th loss in the last 15 games for the once-ranked club.
EKU (21-25) claimed its first victory over UK since the 1999 season and its second over an SEC team this season. The Colonels defeated Mississippi State 16-12 on March 15.
Kentucky returns to action on Friday night, playing host to South Carolina in the first of a three-game series at Cliff Hagan Stadium. First pitch is set for 6:30 p.m. ET.