The Hoosiers' Laren Eustace laced a single to centerfield to drive in Austin Cangelosi for the winning run with one out in the ninth inning. Cangelosi had doubled off UK reliever Zach Strecker (3-2) to begin the decisive inning before the Cats turned to Justin Lewis in hopes of escaping the jam.
Kentucky led 2-1 in the sixth inning before a pair of infield errors helped the Hoosiers tie the game on an RBI single by Scotty Bradley.
The Cats were blanked in the seventh, eighth and ninth innings thanks to some solid bullpen work from IU's B.J. Sabol and Thomas Belcher (4-2), who worked around five hits but walked none over the final frames.
Indiana (29-17) had just seven hits, but leadoff man collected two of them and scored a run for the Hoosiers, who are ranked as high as No. 25 in one of the various baseball polls.
Kentucky (29-20) also struggled offensively with junior outfielder Zach Reks producing the only runs of the day on a third-inning RBI single and a solo home run in the sixth.
The Cats return to action this weekend with a three-game set at Ole Miss. The Rebels are ranked No. 6 in the latest Baseball America Top 25 poll.