Perry, who suffered the injury in the first quarter of Saturday's 45-29 loss, will miss the remainder of the season.
"He didn't take the news very well," U of L coach Charlie Strong said. "He was having a great season, so he was disappointed."
Perry had just two carries for two yards before the injury but leads the team in rushing this season. He has 705 yards and 11 touchdowns on the ground.
Strong said Jeremy Wright, who has 680 yards rushing on the season, and redshirt freshman Corvin Lamb will now share the running back duties. Lamb touched the ball one time on Saturday, resulting in a 53-yard TD catch.
Junior Dominique Brown, who suffered a knee injury in the preseason, has been practicing for over a month and could be used.
"I think we'll be OK in the running game," Strong said. "Senorise was a big part of our offense, but Jeremy Wright will take more of the load.
"If we need to use Dominique, we will."