Defending national champion Louisville is the No. 4 seed in the Midwest Region.
The Cardinals will open the NCAA Tournament on Thursday in Orlando, Fla., against Manhattan and former U of L assistant coach Steve Masiello.
The Jaspers are a No. 13 seed after winning the MAAC Championship recently. Masiello also played for Pitino at Kentucky.
The draw for U of L also puts the Cardinals in the same part of the Midwest Region bracket with top-seeded and undefeated Wichita State, a team the Cardinals defeated in last year's Final Four.
Also in that part of the bracket is Kentucky, the No. 8 seed - and No. 9 seed Kansas State.
The Cardinals (29-5) would face the St Louis-Xavier-N.C. State survivor in the third round. The first two games for U of L will be in Orlando with the Sweet 16 and Elite Eight games to be played in Indianapolis.
Louisville has won 12 of its last 13 games and after steamrolling the AAC Tournament, U of L coach Rick Pitino said his team passed the eye-test to be one of the top four seeds.
But he also said he expected them to be a 2 or a 3 seed. Nobody saw a No. 4 seed coming.
Two hours after the NCAA Tournament bracket was revealed with U of L as the No. 13 team on the NCAA's curve, the Cardinals moved up to No. 3 in the USA Today Coaches poll.
U of L is No. 2 in the Ken Pom rankings and Jeff Sagarin has the Cardinals No. 1 in his predictor rankings.
But the NCAA selection committee disagreed.
Pitino said "we're going to win this thing," noting the AAC conference affiliation hurt his team's ranking with the committee.
The Cardinals are a 15-1 pick by Las Vegas bookmakers to win the title, making them tied for the fifth-lowest odds in the field.
A time and ticket details will be announced later.