Seimone Augustus led the way with 26 points, while Temeka Johnson added 17, Wendlyn Jones and Doneeka Hodges had 15 each and Scholanda Hoston chipped in with 11 off the bench.
The victory for LSU (20-4, 8-2 SEC) earned the program its 16th 20-win season and kept the Lady Tigers in second-place in the Southeastern Conference.
"People stepped up for us today," LSU associate coach Pokey Chatman said. "We had some non-starters come in and contribute in big ways offensively and defensively."
LSU held Florida (16-7, 6-4 SEC) to 35.2 percent shooting and forced 16 turnovers. The Lady Tigers connected on 61.3 percent from the floor, including 61.8 in the second half.
Vanessa Hayden, Florida's leading scorer and rebounder, was held to 17 points and seven rebounds on 7-24 shooting. Florida did outrebound LSU 42-35, but Augustus and Jones each grabbed seven boards.
Johnson, LSU's senior point guard, became the all-time leader in assists at LSU with her fourth of the game. She dished the ball to Hoston for a lay-up with 6:50 left to play in the game. She passed Chatman for the career lead and finished the game with six, giving her 573 career assists.
LSU, which led 42-33 at the break, outscored Florida 13-6 to begin the second half and pulled away from that point, taking as much as a 22-point lead with 1:08 left in the game.
LSU will host Vanderbilt on Thursday at 7 p.m. CST in the Pete Maravich Assembly Center in the first of three straight at home.