The Rebels committed four errors in the first three innings, and the home team jumped on the opportunity to move out to a 6-0 lead before the Rebels tried to fight back.
But it was too little too late, and the Rebels will again call on Sunday starter Scott Bittle to help them win the series.
"We gave them five runs. Credit Florida, but this was probably our most incomplete game of the year," said Ole Miss head coach Mike Bianco. "We made four errors and Phil walked four guys. We don't make the play or turn the double play. It was just a miserable day at the ballpark. Not a very good game for us all the way around. We didn't pitch it. We didn't catch it."
Florida got a run in the first inning after an Ole Miss error, and then topped that with two runs in the second as Ole Miss committed two errors. In the third another Rebel error aided the Gator cause, and it was 6-0 home team after three complete innings.
Zach Miller's two-run homer in the fourth cut the UF lead to 6-2. But the Gators responded with a leadoff triple in the bottom of the fourth by Preston Tucker, which was later followed by a Josh Adams double to make it 7-2.
Ole Miss got two runs in the top of the fifth to cut the Gator advantage to 7-4 and get within striking distance. But in the bottom of the fifth, Mike Mooney doubled and advanced to third on a ground out. He came home on a balk by Rebel reliever Nathan Baker to make it 8-4.
That's the way things ended as Irwin got the loss to fall to 5-3 and Gator starter Stephen Locke (2-0) got the win. Florida outhit Ole Miss 11-8.
Ole Miss fell to 27-11 overall and 10-7 in SEC play, while Florida is now 26-13 and 10-7.
"In the SEC you have to come out every day ready to play. We didn't do that today," Miller said. "We've got another game here, and we need to get ready for that one."
Sunday's third game begins at 11:30 a.m. CT. Senior RHP Scott Bittle (3-2, 1.93 ERA) will start for Ole Miss. Florida has yet to announce a starter.
"As bad as we feel right now, we have to put it behind us," Bianco said. "We've got one game left here, and you always feel better if you win the last one and win two out of three. This is a very good Florida team that wins a lot of games here. They're tough at home. We've got to get off to a better start and put some at-bats together early. We have to play better in the field."