Vanderbilt starter Dean Bennett (1-1) took the loss, allowing four hits and two earned runs in four innings. He also walked three and struck out three.
Austin Peay took a 1-0 lead in the second inning when Frank Punich scored from third when Vanderbilt could not turn a potential inning-ending double play off the bat of Ben Fuller.
The Governors pushed their lead to 2-0 in the third when Chris Hyde's double to center field scored A.J. Ellis.
Austin Peay extended its lead to 5-0 in the fifth off Commodore reliever Jeff Sues. Michael Johnson's two-run home runand a RBI single by Travis Beech completed the Governors' scoring on the night.
Eight of Austin Peay's nine players in the lineup registered hits. Johnson was 2-for-5 while Ellis went 2-for-4.
Vanderbilt's Chris Broadus extended his career-high and team-best hit streak to 11 games, going 2-for-4.
Vanderbilt travels to Auburn this weekend for a three-game Southeastern Conference series. Game times are 6:30 p.m. on Friday and Saturday with the series finale on SUnday beginning at 1:30 p.m.