New Hampshire (9-2-0) scored with just five seconds left in the first period, as Taylor Palazeti recorded her third goal of the season.
NH's Nicole Hekle added the eventual game-winner 2:01 into the second period.
The Badgers got on the board early in the third period, as Jinelle Zaugg scored her 13th goal of the season at the 5:06 mark. But Wisconsin (12-2-0) was stymied from that point on.
New Hampshire netminder Melissa Bourdon had 15 saves, while Wisconsin's Meghan Horras had 21.
The Badgers were out-shot 9-3 in the third period.
Women's basketball loses tournament final
The Blue Demons (5-0) held Wisconsin without a field goal for the first 6:38 of the second half and opened that period on a 15-1 run to build a 50-33 lead.
The Badgers were unable to threaten DePaul from that point on.
Wisconsin shot a season-low 30.6 percent from the field, including 21.4 percent (6 of 28) in the second half. They also turned the ball over 19 times, compared to 11 assists.
Khara Smith paced DePaul with 24 points, 10 rebounds and five steals. Jenna Rubino and Allie Quigley added 17 and 14 points, respectively.
Volleyball to host NCAA matches
The Wisconsin volleyball team is the No. 11 seed for the NCAA Tournament and will host first- and second-round matches at the UW Field House Friday and Saturday.
The Badgers (23-6) will face Loyola (13-17) in a first-round match at 7 p.m. Valparaiso (27-7) and California (18-10) will face each other at 5 p.m. Friday. The winners will advance to a second-round match on Saturday at 7 p.m.
The winner of Saturday's contest at the Field House will qualify for the College Station Regional, hosted by Texas A&M.
Football up in polls
Following its 41-24 win at Hawaii Friday, the Badger football team moved up from No. 24 to No. 21 in the Associated Press poll and from No. 22 to No. 20 in the USA Today coaches' poll.