Jackson, who started in centerfield, batting second in the order, finished the game with two hits in four at-bats, plus a walk, and a run scored. Vitters first appeared in the seventh inning when he pinch hit for Scott Maine, but he would fly out to left field.
Jackson, 24, hit .256 with the Triple-A Iowa Cubs. That included 15 home runs, 47 RBI 22 doubles, 12 triples, and 27 stolen bases.
Vitters, a 22-year-old third baseman, batted .304 in Triple-A, including 17 home runs, 68 RBI, being named a Pacific Coast League All Star.
During Sunday's contest, another one of the Cubs' important future cornerstones, Anthony Rizzo, hit a game-tying solo home run in the ninth inning, yet another encouraging sign for the organization.
In conjunction with the two call-ups, the Cubs send outfielder Tony Campana to the Iowa Cubs. They also shipped utility player Jeff Baker to the Detroit Tigers, receiving two players to be named later.
The Cubs travel to San Diego next, opening a three-game series with the Padres on Monday night.