Bisaccia will replace former Bucs special teams coach Joe Marciano, who took a job in the same capacity with the expansion Houston Texans last month.
Bisaccia served on the Ole Miss staff for three seasons and was promoted to the assistant head coach position before the 2000 season began.
Ole Miss announced Bisaccia's resignation on Monday.
"Rich has been with us from the beginning at Ole Miss and his contributions to our program have been immeasurable," said Ole Miss head coach David Cutcliffe
"He (Bisaccia) is an outstanding coach and person, with a great family. They will be missed here at Ole Miss."
Gruden hired seven assistant coaches on Feb. 25. Click here to view this story.
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