Pierce was the first alternate behind Brian Urlacher, who pulled out of the game after yesterday's disappointing Super Bowl defeat, and Seattle's Lofa Tatupu, who will now start the contest at middle linebacker.
Pierce finished a career-best campaign with 159 tackles, a sack and an interception in 2006.
Barber, who will be making his third consecutive Pro Bowl appearance, will be playing in his final NFL game. He'll once again be a teammate of his brother, Bucs cornerback Ronde.
Jeremy Shockey was also scheduled to participate but pulled out due to various injuries. Shockey, named for the fourth time, also missed last year's contest due to injury.
The Giants Beat Top Stories
Nikita Whitlock's NJ apartment burgalizedThe Giants fullback was subject to a burglary and vandalism a stone's throw away from MetLife Stadium.
The Giants BeatFriday at 8:26 AM
OBJ on bad calls: 'Stevie Wonder can see it'The Giants chatting wide receiver hasn't exactly been thrilled with the officiating this year.
The Giants BeatThursday at 11:20 AM
Cruz ready to 'move on' after McAdoo meetingThe coach and wide receiver had a tee-a-tee this week and according to Victor Cruz all issues were vetted and put on the table.
The Giants BeatWednesday at 1:16 PM
Pierre-Paul out six weeks with sports herniaNo. 90 might not play again this season.
The Giants BeatWednesday at 8:59 AM
Giant Expectations: Week 14 EditionThe Giants Beat discusses what fans of Big Blue are expecting of their team this week against Dallas.
The Giants BeatWednesday at 8:53 AM