Peterson was held out on a "precautionary basis," according to head coach Leslie Frazier because of his sore left ankle.
"He'll be ready to go on Sunday. Just wanted to give him a little more rest prior to the game," Frazier said.
Peterson and Harvin were both listed as probable, meaning a "virtual certainty" to play, on the team's final injury report of the week.
Peterson did "pool work" and other rehabilitation activities Friday, Frazier said, but he wasn't out on the field for the start or end of the Vikings' practice.
"He was a little sore on Wednesday, did alright (Thursday). We were talking this morning: Why not get some rest and get some treatment and (he) should be fresher come Sunday," Frazier said. "He knows everything we've got in the game plan. He's pretty sharp on the things that we were doing Wednesday and Thursday, and even Monday."
Frazier said resting some players, like Peterson and veteran Antoine Winfield (knee), is more of a consideration at this point in the season with the Vikings not yet having their bye week to give their bodies a break.
Peterson leads the league with 775 yards rushing.
Frazier said Thursday he was "pretty convinced" Harvin would be ready for Sunday's game.
Harvin is tied for the league lead with 60 receptions and leads the league with a 35.7-yard average on kickoff returns and 1,274 combined yards (rushing, receiving and returns). His 60 receptions are tied for the franchise record through eight games with Cris Carter's 1994 mark.
Harvin has caught a pass in every game he's played in his NFL career – 55, including the playoffs.
Harvin had been dealing with a hamstring issue that had him limited in Wednesday and Friday's practices, but that wasn't the reason he missed Thursday's practice.
"He moved pretty good. It was a little tight, but he moved pretty good," Frazier said. "He went through everything we did (Wednesday)."
TE John Carlson continued to miss practice after suffering a concussion almost two weeks ago and won't play. He was ruled out for Sunday's game at Seattle.
"I'm hoping that we'll hear some good news when we come back next week. We'd like to get him going," Frazier said. "We want to get him involved in our offense. We think he can really help us. I'm hoping for his sake as well as ours – concussions are a touchy, touchy matter. We're concerned about his health. Hopefully we'll have good news next week.
"He's just not quite there yet."
S Mistral Raymond (ankle) was listed as questionable. If he does play, he won't start because the Vikings are concerned his conditioning isn't at level to handle full-time snaps yet.
Raymond, Winfield and Harvin were all limited in Friday's practice. All others were full participants.
In addition to Harvin and Peterson, the Vikings listed six other players as probable on their injury report – Winfield, DT Fred Evans (knee), DT Letroy Guion (ribs), LB Erin Henderson (elbow), P Chris Kluwe (knee) and QB Christian Ponder (knee).
Tim Yotter is the publisher of Viking Update. John Holler contributed to this notebook. Follow Viking Update on Twitter and discuss this topic on our message boards. To become a subscriber to the Viking Update web site or magazine, click here.